Figure 5.

Localization of tropomyosin and PAT-10 troponin C to the thin filament network in the myoepithelial sheath. Hermaphroditic gonads were dissected from control (a-c and j-l), CeTMI,II,III,IV (RNAi) (d-f and m-o), or pat-10 (RNAi) (g-i and p-r) worms and stained with anti-PAT-10 (a, d, and g), or anti-CeTM (j, m, and p), and anti-actin antibody (b, e, h, k, n, and q). Merged images of PAT-10 or CeTM (green), actin (red), and DNA (blue) are shown in c, f, i, l, o, and r. PAT-10 was expressed in the myoepithelial sheath (MS) but not in the spermatheca (Sp) and colocalized with F-actin (a). CeTM was expressed in both myoepithelial sheath and spermatheca and colocalized with F-actin (j). Bar, 50 μm.