Table 3.
Dissection of the ovulation process by time-lapse recording
Strain | RNAi | Sheath contraction | Spermathecal dilation | Oocyte maturationa | Aberrant cytokinesis | Ovulation | n |
---|---|---|---|---|---|---|---|
Wild-type | Control | 100b | 89 | 100 | 0 | 78 | 9 |
CeTMI,II | 0 | 0 | 100 | 100 | 0 | 5 | |
CeTMI,II,III,IV | 0 | 0 | 100 | 100 | 0 | 10 | |
pat-10 | 0 | 43 | 100 | 71 | 43c | 7 | |
unc-60(r398) | Control | 83 | 83 | 83 | 0 | 83 | 6 |
CeTMI,II | 60 | 100 | 100 | 0 | 80 | 5 | |
CeTMI,II,III,IV | 0 | 80 | 100 | 20 | 60 | 5 | |
pat-10 | 0 | 50 | 83 | 17 | 50 | 6 |
Maturation was characterized by nuclear envelope breakdown
All values except ānā indicate percentages
This includes two cases (29%) of abnormal ovulation, which cleaves the oocyte before completion