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. 2014 Jun 20;16(11):1510–1522. doi: 10.1093/neuonc/nou111

Fig. 7.

Fig. 7.

SP1 is involved in miR-377-induced reduced invasive capability. (A and B) Transwell invasion assay with miR-NC- or miR-377-transduced U87 and U251 cells without transfection or transfected with pEGFP-Sp1 in the presence or absence of mitomycin C. Scale bar = 100 µm (C and D) Invasion of the above cells was quantitatively analyzed. Columns are the average of 3 independent experiments. **P < .01. (E) Western blot analysis of MMP-2 and MMP-9 in the U87 and U251 cells without transfection or transfected with miR-377 or miR-NC. β-actin was used as the loading control. (F) Histological features of brain tumors derived from miR-NC and miR-377-transduced primary invasive cell lines. Scale bar = 200 µm.