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. 2014 Aug 25;16(11):1469–1477. doi: 10.1093/neuonc/nou167

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© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Fig. 1. — Immunocytochemistry (ICC) and immunohistochemistry (IHC) displaying detection limits in HCMV diagnostics. (A) ICC of paraffin-embedded HCMV strain Hi91-infected UKF-NB-4 neuroblastoma cells showing HCMV-IEA positive nuclei in 30%–40% of the cells (arrow). (B) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:1. (C) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:4. (D) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:16. (E) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:64. (F) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:256. (G) ICC of paraffin-embedded HCMV-infected UKF-NB-4 cells diluted with noninfected cells in a ratio of 1:1024. (H) Cerebellar tissue from a patient with HCMV encephalitis showing atypical HCMV-positive nuclei (arrow) of infected neurons and positive nuclei of surrounding glial cells (arrow-heads). (Scale 100 µm).