Figure 2.

Dual specificity tyrosine-phosphorylation-regulated kinase 1A mediated phosphorylation of GluN2A at S¹⁰⁴⁸ increases the surface expression of GluN2A. COS-7 cells were transiently co-transfected with plasmids to express GluN1 and wild-type (wt), or mutant versions of GFP-GluN2A (S1048A, phospho-deficient; S1048E, phospho-mimetic), in the presence or absence of HA-DYRK1A (wt, wild-type; KD, kinase-inactive). After fixing, the cells were incubated with an anti-GFP antibody to label the surface receptors (blue). Direct green fluorescence was used to measure total GFP-GluN2A expression (green). HA-DYRK1A expressing cells were identified by anti HA-immunostaining (red). Scale bar = 10 µm. The histogram represents the mean ± SEM of the GluN2A surface expression normalized to the total GFP-GluN2A signal, with the values for transfections GluN1+GFP-GluN2Awt considered as 100 (n = 38–130 cells from, at least, three independent experiments per condition; *p < 0.05, ***p < 0.001, ANOVA).