Figure 11.

Postendocytic and steady-state retrieval of Tac-furin/ADA back to the plasma membrane after internalization. (A) A portion of Tac-furin/ADA is retrieved back to the plasma membrane after internalization. Cells were incubated with Ax488–anti-Tac for 5 min, washed and chased for 2, 5, 15, and 30 min in the presence of the quenching antibody anti-Ax488. (B) Sorting of Tac-furin/ADA from the TGN back to the plasma membrane at steady state is inhibited. Cells were incubated with Ax488–anti-Tac for 15 min, washed and chased for 2 h. Labeled cells were further incubated in the presence of anti-Ax488 for 5, 15, 30, 60, 90, and 120 min. In both sets of experiments, cell-associated fluorescence was quantified as described in MATERIALS AND METHODS. Data points represent the means of two independent experiments. Error bars indicate SEM.