Figure 9.

Internalized Tac-furin/ADA starts to segregate from the ERC into the TGN as early as 15 min after being internalized. Cells were incubated with Ax546-anti-Tac (A and E) and Ax488–transferrin (B and F) for 5 min, washed, and chased for 10 min in the presence of Ax488-transferrin. C and G show the merge of the anti-Tac signal (red) and the transferrin signal (green). A–C and E–G show single confocal planes. A projection of successive confocal planes is shown in D and H. Colocalized areas range from orange to yellow. Bar, 10 μm.