Fig. 2.

Indirect inhibition of [³H]CCK-8 accumulation by PMA in human SCH. (A) Time-dependent effect of PMA pretreatment on [³H]CCK-8 accumulation in human SCH. Human SCH were pretreated with PMA (0.1 and 1 μM) for various times (10–60 minutes). [³H]CCK-8 accumulation (1 μM, 3 minutes) was expressed as a percentage of the accumulation measured in vehicle control–treated cells. (B) [³H]CCK-8 accumulation in the presence of vehicle control, PMA, or BIM I at indicated concentrations. [³H]CCK-8 accumulation (1 μM, 3 minutes) in human SCH was expressed as a percentage of the accumulation measured in the absence of PMA or BIM I. Data represent mean ± S.E.M. from n = 3 donors in triplicate. *P < 0.05 versus vehicle control treatment by one-way analysis of variance, followed by Dunnett’s t test. Transport experiments were performed on day 5 or 6 of culture.