Fig. 4.

Relative quantitation analysis of multiple gene targets was performed to evaluate the specificity of CYP3A4 siRNA treatment on multiple drug-metabolizing enzymes. The data displayed represent the average changes observed from four donors after treatment of CYP3A4 siRNA for 72 hours. Triplicate samples of both vector control and siRNA-treated cells were combined and analyzed in duplicate (siRNA-treated/vector control treatment); mean values displayed from n = 4 donor treatments, each containing n = 3 interassay pooled replicates, n = 2 intra-assay replicates. The data demonstrate that CYP3A4 siRNA was highly specific toward reducing mRNA levels of CYP3A4 (∼90%) when compared with the vector control mRNA levels. No other drug-metabolizing enzyme (including CYP2C9 and UGT2B7) was affected by CYP3A4 siRNA treatment.