Fig. 5.

Quantitation of protein levels of CPR, CYP2C9, and CYP3A4 in HepatoPac donor 2 was achieved using ELISA by pooling n = 24 samples, of donor 2, and measuring n = 2 intra-assay replicates. Concentrations were compared with literature reported (Gan et al., 2009; Ohtsuki et al., 2012) and those generated in the same assay for a single HLM donor. The levels of CPR, CYP2C9, and CYP3A4 protein present in the HepatoPac model were consistent with those present in a single donor of HLM and in literature reports. There was no change in CPR or CYP2C9 content from treatment of the HepatoPac model with siRNA-targeting CYP3A4. The content of CYP3A4 protein decreased by 2.1-fold after treatment with siRNA-targeting CYP3A4.