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. 2014 Nov;86(5):561–569. doi: 10.1124/mol.114.092544

Fig. 2.

Fig. 2.

Ribonucleotide reductase and 14-3-3σ are upregulated in G3K cells. (A) Western blot analysis of hENT1, RRM1 and 2, 14-3-3σ, ζ, and θ expression in both MiaPaCa-2 and G3K cells. (B) Real-time RT-PCR analysis of RRM1 and 14-3-3σ mRNA in MiaPaCa-2 and G3K cells (n = 3; P < 0.001). (C) Western blot analysis of RRM1 and 14-3-3σ in the intermediate gemcitabine-resistant cells G100, G500, and G1K. Actin was used as a loading control for Western blot analyses, and glyceraldehyde-3-phosphate dehydrogenase was used as an internal control for PCR analyses.