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. 2014 Nov;86(5):561–569. doi: 10.1124/mol.114.092544

Fig. 3.

Fig. 3.

14-3-3σ upregulation associates with and contributes to gemcitabine and Ara-C resistance. (A) 14-3-3σ knockdown reduces gemcitabine and Ara-C resistance in G3K cells. G3K cells were transiently transfected with 14-3-3σ siRNA (Si) or scrambled control siRNA (Scr) followed by Western blot analysis of 14-3-3σ expression and MTT assay of cellular response to gemcitabine and Ara-C. RRF = IC50(Si or 14-3-3σ)/IC50(Scr or Vec) (n = 3–4; ***P < 0.001). (B) 14-3-3σ knockdown reduces gemcitabine resistance in G500 cells. G500 cells were transiently transfected with 14-3-3σ siRNA or scrambled control siRNA followed by Western blot analysis of 14-3-3σ expression and MTT assay of cellular response to gemcitabine (n = 4; **P < 0.01). (C) 14-3-3σ stable knockdown reduces gemcitabine resistance in G3K cells. G3K cells stably transfected with 14-3-3σ shRNA (Sh) or scrambled control (Scr) were established and subjected to Western blot analysis and MTT assay of cellular response to gemcitabine (n = 3; ***P < 0.001). (D) 14-3-3σ overexpression in MiaPaCa-2 cells causes gemcitabine and Ara-C resistance. Stable MiaPaCa-2 cells with 14-3-3σ overexpression (14-3-3σ) or transfected with vector control (Vec) were established and subjected to Western blot analysis and MTT assay of cellular response to gemcitabine and Ara-C (n = 3–4; ***P < 0.001). (E) Association of 14-3-3σ expression with gemcitabine resistance. Expression of 14-3-3σ and IC50 to gemcitabine in MiaPaCa-2, G3K, and the revertant G3K/REV cells was determined using Western blot analysis and MTT assay, respectively (n = 4; **P < 0.01).