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. 2014 Nov;86(5):561–569. doi: 10.1124/mol.114.092544

Fig. 5.

Fig. 5.

Effect of Uhrf1 and DNMT1 on 14-3-3σ expression. (A) Western blot analysis of Uhrf1, DNMT1, DNMT3a, and p21 expression as well as real-time RT-PCR analysis of DNMT3b expression in MiaPaCa-2, G3K, and G3K/REV cells. (B–D) Effect of Uhrf1, DNMT1, DNMT3a, and DNMT3b knockdown on 14-3-3σ expression. MiaPaCa-2 cells were transiently transfected with scrambled control siRNA (Scr) or siRNAs (Si) targeting Uhrf1 (B), DNMT1, DNMT3a, and DNMT3b (D) followed by Western blot analysis of Uhrf1, DNMT3a, and 14-3-3σ or real-time RT-PCR analysis of DNMT3b and 14-3-3σ (B–D) (n = 4–5; *P < 0.05; **P < 0.01; ***P < 0.001). Actin and glyceraldehyde-3-phosphate dehydrogenase were used as a loading control for Western blot and internal control for PCR analyses, respectively.