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. 2014 Nov;86(5):561–569. doi: 10.1124/mol.114.092544

Fig. 6.

Fig. 6.

Binding of Uhrf1 and DNMT1 to the CpG island of the 14-3-3σ gene. (A) ChIP analysis of Uhrf1 binding to the CpG island sequence or non-CpG island sequence of the 14-3-3σ gene. (B and D) Effect of Uhrf1 knockdown on Uhrf1 and DNMT1 binding to the CpG island sequence of the 14-3-3σ gene in MiaPaCa-2 cells. MiaPaCa-2 cells were transiently transfected with scrambled control (Scr) or Uhrf1 siRNAs (Si) followed by ChIP analysis of Uhrf1 and DNMT1 binding. (C) DNMT1 binding to the CpG island of the 14-3-3σ gene in G3K cells is less than that in MiaPaCa-2 cells. ChIPs with histone H3 antibody or without any primary antibody were used as positive and negative controls, respectively. Normal IgG was also used as a negative control, which did not immunoprecipitate any DNA (data not shown). Ab, antibody.