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. 2014 Nov;86(5):570–579. doi: 10.1124/mol.114.092163

Fig. 3.

Fig. 3.

Rexinoids inhibit cytokine expression after stimulation with AII. Cells were pretreated for 24 hours with bexarotene or 9-cis retinoic acid and stimulated with AII for 3 hours (mRNA) or 48 hours (protein). (A) Total RNA was isolated and qRT-PCR conducted for MCP-1 mRNA. Shown are mRNA absolute copy number normalized to GAPDH. Mean ± S.E.M., n = 3 independent experiments. (B) Conditioned media was collected and assayed for MCP-1 protein levels normalized to total protein. Shown are mean ± S.E. *P < 0.05 versus DMSO.