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. 2014 Oct 17;5:548. doi: 10.3389/fmicb.2014.00548

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Copyright © 2014 Neumann, Fraiture, Hernàndez-Reyes, Akum, Virlogeux-Payant, Chen, Pateyron, Colcombet, Kogel, Hirt, Brunner and Schikora.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screen for Salmonella proteins that induce HR-like symptoms in plants. SVF and effector genes were cloned into plant expression vectors and expressed as HA- and Myc-tagged versions in N. benthamiana leaves via Agrobacterium-mediated transformation. Symptoms were observed 5 days after infiltration. Expression of eight proteins resulted in macroscopic changes in leaf morphology when compared to the non-transformed parts of the leaf (control), transformation with Agrobacterium GV3101 or infiltration with 10 mM MgCl₂. The experiment was repeated 4 times with both versions of bacterial fusion protein. Only the right side of the leaf was infiltrated.