Figure 2. Effects of FST transfection on PI3K/Akt/mTOR pathway gene expression.

(A) Relative mRNA expression levels of PI3K, Akt, mTOR, S6K, FoxO1 and MuRF1 in transfected cells compared with control cells at 24 h, Duck β-actin and GAPDH were used as the internal controls. The data were analysed by ANOVA and Tukey's test. The label ‘*’ indicates a significant difference (P<0.05), and ‘**’ indicates a significant difference (P<0.01). The results are presented as the mean±S.E.M. (n=3). (B) and (C) The expression of Akt, phospho-Akt (Thr³⁰⁸) (p-Akt), mTOR, phospho-mTOR (serine 2448) (p-mTOR), S6K, phospho-S6K (Ser⁴¹⁷) (p-S6K), FoxO1, phosphor-FoxO1 (Ser²⁵⁶) (p-FoxO1) proteins were detected by Western blot after transfection for 24 h. Each treatment and each sample were repeated in triplicate, the expression of eight proteins were normalized to the equal a value of 1 in control, *represents a significant difference (P<0.05).