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. 2014 Oct 17;34(5):e00143. doi: 10.1042/BSR20140085

Figure 5. Effects of rapamycin and duck FST overexpression on myoblast proliferation.

Figure 5

(A) The relative mRNA expression of FST, including control group, treatment with rapamycin for 24 h (designated as rapamycin group), transfected with pEGFP-duFST for 24 h after 24 h rapamycin treatment (designated as rapamycin+pEGFP-duFST group), the data were analysed by ANOVA and Tukey's test. The label ‘*’ indicates a significant difference (P<0.05), and ‘**’ indicates a significant difference (P<0.01). The transfection efficiency of rapamycin+pEGFP-duFST was observed using a fluorescence microscope (×100). (B) Representative images of myoblasts for three different treatment groups (×100). (C) Proliferation myoblasts was measured using an MTT assay; data are presented as the mean±S.E., n=5 wells, *(P<0.05), **(P<0.01). (D) The number of BrdU–FITC-labelled nuclei per 100 DAPI-labelled nuclei. (E) BrdU-labelled nuclei for different treatment group; all of the nuclei are labelled blue by DAPI, but only the proliferating nuclei are labelled green by monoclonal anti-BrdU (×200), the arrows shows the proliferation cells.