Table 1. Primer sequences used for qRT-PCR.
F and R are forward and reverse primers, respectively.
| Gene | Primer sequence (5′-3′) | Product size (bp) | Tm (°C) |
|---|---|---|---|
| FST | F: ACAACTTACCCAAGCGAGTGTG | 145 | 58 |
| R: CATCTTCCTCTTCTTCCTCTGG | |||
| PI3K | F: CTTTTACCGAGGAGGTTCTGTGG | 137 | 60 |
| R: CTGAAGGTTGGTCTTTGTGGAC | |||
| Akt | F: TCTTTGCTGGCATTGTTTGGC | 152 | 60 |
| R: GCTGTCATCTTGGTCAGGAGGAGT | |||
| mTOR | F: CTATCTGCCTCAGCTCATTCCT | 121 | 60 |
| R: GTCATCCAGGTTAGCTCCAAAG | |||
| FoxO1 | F:AGGTTCACCAAATCCAGACTACAG | 182 | 60 |
| R: GCGTTGTGCGGAGGAGAATCAG | |||
| S6K | F: ATAATCGTGCTGTGGACTGGTG | 155 | 60 |
| R: TCTGGCTTCTTGTGTGAGGTAGG | |||
| MuRF1 | F: TCAACATCTACTGCGTCACCTG | 128 | 57 |
| R: GCTATTCAACTCGCTCTTCTGG | |||
| MSTN | F: GCACTGGTATTTGGCAGAGTATT | 142 | 60 |
| R: TCACCTGGTCCTGGGAAAGT | |||
| ACVR2 | F: CCGATTTTGGTCTAGCTGTACG | 143 | 60 |
| R: GTCTATTCTCAGGAAGGCGTCTC | |||
| β-actin | F: GCTATGTCGCCCTGGATTTC | 168 | 60 |
| R: CACAGGACTCCATACCCAAGAA | |||
| GAPDH | F: AAGGCTGAGAATGGGAAAC | 254 | 60 |
| R: TTCAGGGACTTGTCATACTTC |