Fig. 2.

Effect of three kinds of MMP inhibitors on TNF-α secretion in LPS-stimulated BV2 microglial cells. (A) Effect of MMP inhibitors on TNF-α secretion in conditioned media and on TNF-α expression in cell lysates. BV2 microglial cells were incubated with LPS (100 ng/ml) for 3 h in the absence or presence of NNGH, M8I, or M9I. Cell lysates were prepared by passage through a 26-gauge needle, and the production of TNF-α in conditioned media and cell lysates was determined by ELISA. The average values are indicated. (B) The percent release of TNF-α was determined by dividing the amount of TNF-α in the supernatant by the total amount of TNF-α (cell-associated + secreted TNF-α). The data are expressed as the mean ± S.E.M. n=4–6 per group. *p<0.05 vs. LPS-treated cells.