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. 2014 Sep 30;22(5):390–399. doi: 10.4062/biomolther.2014.062

Fig. 6.

Fig. 6.

Effects of TI-I-174 on transcriptional activation of AP-1 and NF-κB in RAW 264.7 macrophages. (A) Cells were transiently cotransfected with pAP1-Luc plasmid and Renilla reporter gene as described previously. After 24 h of culture, cells were pretreated with indicated concentration of TI-I-174 for 1 h followed by incubation with 100 ng/ml LPS for additional 8 h. Transcriptional activity of AP-1 was assessed by luciferase reporter assay as described previously. Values are expressed as fold increase relative to control cells, mean ± SEM (n=3). *p<0.05 compared with the cells not treated with LPS; #p<0.05 compared with cells treated with LPS. (B) Cells were co-transfected with pGL4/NF-κB plasmid and Renilla reporter gene. After 24 h incubation, cells were pretreated with TI-I-174 for 1 h followed by stimulation with 100 ng/ml LPS for additional 8 h. NF-κB activity was measured by luciferase assay and values are expressed as fold increase relative to control cells, mean ± SEM (n=3).