FIG. 1.

(A) Location of C. albicans hypha-defective Cdc42p point mutations modeled onto the crystal structure of human Cdc42p. Side chains of mutated amino acids are depicted in black; the Rho insert domain and effector domain are highlighted in grey. The image was generated from PDB file 1AN0 of the Cdc42Hs-GDP complex using RasMol version 2.6. (B) Cdc42p mutants were defective in hypha formation on solid Spider medium. Strains AV05 (cdc42-S158T), AV03 (cdc42-E100G), AV02 (cdc42-S71P), AV06 (cdc42-S26I), CaDH50, and Sc5314 were grown for 3 to 4 days at 30°C. (C) Cdc42p mutants had defects in hypha formation in 10% FBS. Strains (see Fig. 2B) were grown in minimal medium plus 10% FBS for 4 h at 37°C. Bar, 10 μm. The scale bar applies to all three images in the bottom panel.