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cdc42-S26I and cdc42-E100G mutants had a reduced ability to cause EC damage. Strains CAI-12, AV15 (CDC42/cdc42), AV08 (cdc42-S26I), and AV09 (cdc42-E100G) were incubated for 3 h with human ECs. The percentage of EC damage was measured with a chromium release assay. Results are the averages ± standard deviations of triplicate measurements. Asterisks indicate a P value of <0.005 compared to that for cells infected with CAI-12, as determined by the Wilcoxon rank sum test.
