TABLE 2.
Primers used
| Primer | Sequencea | Sequence targetedb |
|---|---|---|
| 465 | CGTCCTAAATCTATTTTTATTG | 5′ CDC42 |
| 466 | GCCCAACACATCAAACGAGATC | 3′ CDC42 |
| 641 | CGAACTTGGTAGTGGGACCGAATGG | 3′ CDC42 |
| 616 | GCAACAACCCCATACACACTGG | URA3 |
| 646 | CCTCTTTATCCTGTCTGAACCGG | HisG |
| 499 | GCATGGATATTCCATGTCAAGG | 5′ CDC42 |
| 601 | GATTACGACAGATTACGGCCGTTGCCATATCCATCGACTG | cdc42-S71P |
| 602 | CAGTCGATGGATATGGCAACGGCCGTAATCTGTCGTAATC | cdc42-S71P |
| 605 | CGTATACCACTATTAAATTTCCAGC | cdc42-S26I |
| 606 | GCTGGAAATTTAATAGTGGTATACG | cdc42-S26I |
| 607 | GAAAAATGGTTCCCCGGGGTTCATCACCATTGTCC | cdc42-E100G |
| 608 | GGACAATGGTGATGAACCCCGGGGAACCATTTTTC | cdc42-E100G |
| 638 | GTATGTTGAGTGCACTGCATTGACTCAAAG | cdc42-S158T |
| 639 | CTTTGAGTCAATGCAGTGCACTCAACATAC | cdc42-S158T |
a
Underlined nucleotides are those changed from wild type to alter amino acids.
b
This represents either the region of the gene where the primer anneals or the introduced mutation.