TABLE 3.

Hyphal, pseudohyphal, and budded cells in cdc42 mutants

Strain	CDC42 allele	Growth condition	% of totala
			Hyphae	Pseudohyphae	Budded
Sc5314b	CDC42	10% FBS	99	1	0
CaDH50b	CDC42	10% FBS	86	2	12
AV05	S158T	10% FBS	17	51	32
AV09	E100G	10% FBS	2	13	85
AV06	S26I	10% FBS	3	38	59
Sc5314	CDC42	Spider	60	16	24
CaDH50	CDC42	Spider	46	23	31
AV05	S158T	Spider	0	17	83
AV09	E100G	Spider	0	8	92
AV06	S26I	Spider	0	5	95

^aCells were counted for the proportion of buds versus hyphae using DAPI to stain DNA and Calcofluor White to stain the cell wall, in order to ensure that counts reflected the true number of individual cells and that pseudohyphal constrictions would be accentuated by the Calcofluor stain. After 4 h of growth, categories of cells counted were as follows; hyphae were long, filamentous cells with parallel sides and no constrictions visible between cells; pseudohyphae were at least twice the length of a budded cell, with constrictions visible at the cell junctions, or were long filamentous cells with nonparallel sides. In all categories, only nucleated cells (n = 200) were counted.

^bSc5314 is a clinical isolate, while CaDH50 is the parental laboratory strain. Both are CDC42/CDC42.