TABLE 4.
Actin localization in cdc42 mutant cells
| Strain | CDC42 allele | Growth conditionsa | Actin localization (% of cells)b
|
||
|---|---|---|---|---|---|
| Random | Polar patches | Polar and cables | |||
| AV03 | E100G | 30°C | 67 | 33 | <1 |
| CaDH50 | CDC42 | 30°C | 25 | 65 | 10 |
| AV03 | E100G | 37°C | 82 | 17 | <1 |
| CaDH50 | CDC42 | 37°C | 7 | 84 | 9 |
| AV03 | E100G | 37°C + serum | 82 | 17 | 1 |
| CaDH50 | CDC42 | 37°C + serum | 0 | 55 | 45 |
| AV06 | S26I | 37°C + serum | 31 | 69 | <1 |
| AV05 | S158T | 37°C + serum | 47 | 49 | 4 |
a
A total of 300 rhodamine-phalloidin-stained cells were counted after 4 h of growth at the temperature indicated in minimal medium (+/− 10% FBS).
b
Random, no polarized actin patches; polar patches, polarized actin patches with no actin cables visible; polar and cables, polarized actin patches and actin cables visible. For details, see text.