Fig. 3.

Effects of ascorbic acid and deprenyl on l-dopa induced decreases in TPH⁺ cell counts. TPH⁺/NeuN⁺ cell bodies were counted within the caudal-extent of the dorsal DRN subregion. (A) Both ascorbic acid (400 mg/kg) and deprenyl (2 mg/kg) prevented the effects of l-dopa at decreasing TPH⁺/NeuN⁺ cell bodies (*P < 0.05 compared with vehicle, two-way ANOVA and Tukey’s post-hoc test). (B) Representative DRN images (original magnification: 20×) illustrating TPH⁺/NeuN⁺ colabeling (green = NeuN⁺, red = TPH⁺, yellow = TPH⁺/NeuN⁺ colabel) (n = 4–6 per group).