FIG. 3.

hph1Δ hph2Δ cells are sensitive to growth in conditions of high pH and high NaCl and with Calcofluor White and Congo Red. Wild-type (WT, BY4741), hph1Δ (strain 1621), hph2Δ (strain 380), and hph1Δ hph2Δ (strain VHY60) strains were grown to stationary phase in YPD. Cultures were then diluted to an optical density at 600 nm of 0.5, and fivefold serial dilutions were spotted on YPD plates with no addition, 50 mM Tris-HCl at pH 8.8, 50 mM MES at pH 5.5, 1 M NaCl, 2 mg of Congo Red per ml, or 0.188 mg of Calcofluor White per ml. Plates were incubated at room temperature for 3 days for YPD and pH 5.5, 4 days for pH 8.8, Calcofluor White, and Congo Red, and 5 days for NaCl.