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. 2014 Oct 15;28(20):2219–2232. doi: 10.1101/gad.247163.114

Figure 4.

Figure 4.

Arid3a OE promotes incorporation of ES cells into the TE of developing embryos, whereas its loss leads to defective placentas. (A) ZsGreen-Arid3a-overexpressing TS-like cells integrate into the TE layer of developing embryos, whereas Arid3a−/−-null (knockout [KO]) ES cells remain within the ICM layer of ex vivo embryos. The four- to eight-cell stages of embryos injected with control ES cells and Cdx2-overexpressing cells served as negative and positive controls, respectively. (B) Placentas of E11.5 Arid3a−/− embryos are defective. IHC was performed with anti-proliferin, which marks TGCs, and anti-Tpbpa, which stains SpTs. (D) Deciduum. Anti-Arid3a IHC revealed high levels of Arid3a expression in Arid3a+/+ TGCs and SpTs. The dotted lines denote the wild-type TGC and SpT expression domains that are grossly disorganized in the null placentas.