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. 2014 Oct 15;28(20):2261–2275. doi: 10.1101/gad.250449.114

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© 2014 Chen et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Tat recruits Ssu72 to the integrated HIV-1 proviral promoter in 2D10 T cells. (A) Immunoblot analysis of Tat and cellular protein expression in TNF-α treated 2D10 cells for the indicated times. (B) Immunoblot analysis of protein expression of 2D10 cells transfected with Ssu72 or Cyclin T1 siRNAs with the indicated antibodies. (C) ChIP analysis of the recruitment of virus-encoded Tat and host cell factors to the integrated provirus in TNF-α-induced 2D10 cells. Schematic representation of the genomic organization of the lentiviral vector and the relative locations of the primers used for ChIP are shown at the top of the panel. (D) Model depicting three different steps regulated by Tat to facilitate the transition from promoter clearance to early elongation at the HIV-1 promoter. See the Discussion for details.