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. 2014 Oct 15;28(20):2276–2290. doi: 10.1101/gad.248492.114

Figure 6.

Figure 6.

Human FAN1 lacking a UBZ domain cannot incise the 5′ flap or nicked HJ in the presence of the ID complex. (A) Surface representation of PaFAN1 bound to DNA. (B) Surface representation of the model of ID bound to DNA. The model was based on data from Joo et al. (2011) (Protein Data Band [PDB], 3S4W and 3S4Z). (C) Nuclease activities of human FAN1 lacking a UBZ domain (lanes 1–12, 25 nM; lanes 13–17, 250, 500, 2500, 10,000 nM) on 5′ flap DNA (5 nM) (Supplemental Fig. 8N) were examined in the presence of 25 nM human ID complex and quantified. The assay was also performed in the presence of 25 nM hMus81–Eme1 complex instead of the ID complex. Assay buffer condition was the same as that used in Figure 1C. (D) Nuclease activities of human FAN1 lacking a UBZ domain (25 nM) on nicked HJ DNA (5 nM) were examined in the presence of 25 nM human ID complex.