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. 2014 May 27;23(21):2649–2660. doi: 10.1089/scd.2014.0013

FIG. 2.

FIG. 2.

In situ hybridization for placental differentiation marker genes. At E10.5 individual Cx31.1−/− placentas show absence of Tpbpa-positive precursors in the spongiotrophoblast (SPT) layer (B), whereas other Cx31.1−/− littermates show Tpbpa-positive cells but a reduced SPT layer (C) compared with Cx31.1+/+ controls (A). The formation of secondary TGC derived from the SPT cells is reduced. Compared with controls there are less Prl3d/Pl-1 and Prl2c-positive TGC in the junctional zone, and the TGC layer appears thinner in the Cx31.1−/− placentas compared to controls (D, E, F, G, H, I). Prl3b1/Pl-2-positive TGC are missing in the Tpbpa-negative placentas (B, K) compared to Tpbpa positive placentas (J, L) and numbers of Ctsq-positive TGC are reduced in Cx31.1−/− placentas compared with controls (M, N, O). Scale bars represent 100 mm. Insets show higher magnification of stained areas. D, decidua; L, labyrinth; S, SPT; arrowheads, TGC.