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. 2014 May 27;23(21):2649–2660. doi: 10.1089/scd.2014.0013

FIG. 3.

FIG. 3.

In situ hybridization for placental transcription factors. There are no obvious differences in the amount or localization of Hand1 (A–C), Ascl2 (D–F), or Gcm1 (G–I)-positive trophoblast cells in Cx31.1−/− placentas compared to controls. Ki67 staining (J, K) and quantification of Ki67-positive cells (L) reveal no differences in the number of proliferating cells in Cx31.1−/− placentas. TUNEL assay reveals only basal apoptosis levels (arrows) in Cx31.1−/− (N, O), and control placentas (M). Scale bars represent 100 μm in A–I; 200 μm in J–O. Insets show higher magnification of stained areas. D, decidua; L, labyrinth; S, SPT; arrowheads, TGC.