TABLE 2.
Genotype of strain mixed with the a2 b1 oligomycin- resistant strain | cAMP added (mM) | Cytoduction frequencya | % of wild-type frequency |
---|---|---|---|
a1 b1 Hygr (positive control) | None | 2.3 × 10−5 | 100 |
a2 b1 Hygr (negative control) | None | < 6.1 × 10−8 | <0.27b |
a1 b1 Δgpa3 | None | 3 × 10−7 | 1.3 |
a1 b1 Δbpp1-2 | None | 2.5 × 10−6 | 11 |
a1 b1 Δgpa3 Δbpp1-2 | None | 5.4 × 10−7 | 2.3 |
a1 b1 Hygr | 6 | 3.4 × 10−5 | 100 |
a2 b1 Hygr | 6 | < 9 × 10−8 | <0.26b |
a1 b1 Δgpa3 | 6 | 4.0 × 10−5 | 118 |
a1 b1 Δbpp1-2 | 6 | 4.3 × 10−5 | 126 |
a1 b1 Δgpa3 Δbpp1-2 | 6 | 3.1 × 10−5 | 91 |
Proportion of hygromycin- and nourseothricin-resistant colonies that were also oligomycin resistant.
No cytoductants detected.