TABLE 3.
Conjugation tube formationa
| Strain stimulated with pheromone | cAMP added (mM) | Total no. of cells counted | % with conjuga- tion tubes |
|---|---|---|---|
| FB2 | 0 | 320 | 82 |
| FB2 | 6 | 351 | 17 |
| FB2Δgpa3 | 6 | 319 | 18 |
| FB2Δbpp1-2 | 6 | 316 | 17 |
| FB2Δfuz7 | 6 | 371 | 0 |
| RK1786b | 6 | 382 | 0 |
| FB1 | 0 | 361 | 74 |
| FB1gpa3QL | 0 | 1,017 | 17.5 |
| FB1gpa3QLΔbpp1-2 | 0 | 709 | 18 |
a
Cells were treated for 5 h with synthetic a1 pheromone (FB2 and derivatives) or a2 pheromone (FB1 and derivatives). Conjugation tube formation was assessed microscopically.
b
RK1786 is derived from FB1 and carries a Tn5H insertion in the pra1 gene.