Figure 2.

Agam\Or13 and Agam\Or15 differ in their responsiveness to 4-methylphenol and acetophenone. (A) Concentration–response relationships for activation of Agam\Or13 + Agam\Orco by acetophenone (Ace) and 4-methylphenol (4-mp). Current responses to each concentration of odorant (n = 5) were normalized to the response of the same oocyte to 5 µM Ace. EC₅₀ values for Ace (6.7±1.8 µM) and 4-mp (45±19 µM) were significantly different (P < 0.01, t-test). Maximal response values for Ace (3.4±0.2) and 4-mp (4.9±0.5) were not significantly different (P = 0.077, t-test). (B) Concentration–response relationships for activation of Agam\Or15 + Agam\Orco by acetophenone (Ace) and 4-methylphenol (4-mp). Current responses to each concentration of odorant (n = 4) were normalized to the response of the same oocyte to 30 µM Ace. EC₅₀ values for Ace (18±9 µM) and 4-mp (71±12 µM) were significantly different (P < 0.01, t-test). Maximal response values for Ace (1.9±0.2) and 4-mp (0.64±0.03) were significantly different (P < 0.05, t-test). (C) An oocyte expressing Agam\Or13 + Agam\Orco was challenged with challenged 20 s applications of 1mM 4-mp and 1mM Ace. (D) An oocyte expressing Agam\Or15 + Agam\Orco was challenged 20 s applications of 1mM 4-mp and 1mM Ace.