Figure 1.

Histological analysis of RGC survival following ONC injury. Representative images from cross-sectioned globes 3 weeks following ONC (A–D) and uninjured sham nerve exposure (E–H). The RGC counts were obtained from sections across the optic nerve head for EYFP- and Brn3a-expressing cells (I). The average number of cells and standard deviation are shown. Sectioned retinas were immunostained with anti-GFP (green) and anti-Brn3a (red) antibodies. The EYFP fluorescence is enhanced using anti-GFP antibodies.