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. 2014 Oct 1;9(10):e108962. doi: 10.1371/journal.pone.0108962

Figure 4. Molecular and morphological phenotypes of the PpPPR_63 KO and complementation mosses.

Figure 4

(A) Schematic diagram of PpPPR_63 and construction of the KO locus. The nptII cassette was inserted into the 4th intron of the PpPPR_63 gene. (B) Detection of PpPPR_63 transcripts by RT-PCR. (C) Two-week-old protonemal colonies of the PpPPR_63 KO line (Δ63) and the complementation lines (F63 and M63). Bars  = 5 mm. (D) One-week-old filamentous protonemata regenerated from cut leaves. (E) Steady-state levels of nuclear-encoded tRNAs.