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. 2014 Jul 31;9(10):2326–2333. doi: 10.1021/cb500463f

Table 1. Summary of Kinetic Data for dCK Library A.

		l-Thy			d-Thy			l-Thy/d-Thy
variant	mutations	K_M (μM)	k_cat (s^–1)	k_cat/K_M (s^–1 M^–1 × 10^–3)	K_M (μM)	k_cat (s^–1)	k_cat/K_M (s^–1 M^–1 × 10^–3)	k_cat/K_M fold-change
A0	A100V, R104M, D133S	10.2 ± 1.1	1.41 ± 0.03	137	11.7 ± 1	3.49 ± 0.1	298	0.5
A1	A0 + F96D	na	na		na	na
A2	A0 + W58E	na	na		na	na
A3	A0 + E196L	na	na		na	na
A4	A0 + F96Y	489 ± 40	0.62 ± 0.03	1.2	1121 ± 146	1.70 ± 0.14	1.5	0.8
A5	A0 + W58V	598 ± 89	0.55 ± 0.06	0.9	1254 ± 347	0.84 ± 0.19	0.7	1.3
A6	A0 + E196A	34.4 ± 4.5	3.01 ± 0.13	87	53.4 ± 7.5	3.14 ± 0.16	59	1.5
B0	R104M, D133N	18.5 ± 4.3	1.61 ± 0.11	87	18.5 ± 3.1	1.49 ± 0.06	81	1.1