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. 2014 Jul 31;9(10):2326–2333. doi: 10.1021/cb500463f

Table 2. Summary of Kinetic Data for dCK Library B.

		l-Thy			d-Thy			l-Thy/d-Thy
variant	mutations	K_M (μM)	k_cat (s^–1)	K_cat/K_M (s^–1 M^–1 × 10^–3)	K_M (μM)	k_cat (s^–1)	K_cat/K_M (s^–1 M^–1 × 10^–3)	K_cat/K_M fold-change
B0	R104M, D133N	18.5 ± 4.3	1.61 ± 0.11	87	18.5 ± 3.1	1.49 ± 0.06	81	1.1
B1	B0 + P89F	na	na		na	na
B2	B0 + A138I	na	na		na	na
B3	B0 + L102Y	20.9 ± 2.2	2.69 ± 0.11	129	20.4 ± 2.6	2.59 ± 0.12	127	1
B4	B0 + M85Y	53.9 ± 5.3	1.73 ± 0.05	32	47.8 ± 22.2	1.34 ± 0.41	28	1.1
B5	B0 + V55E	1145 ± 153	1.29 ± 0.12	1.1	655 ± 96	0.67 ± 0.04	1	1.1
B6	B0 + L191A	48.9 ± 6.4	1.92 ± 0.08	39	30.3 ± 5.2	0.69 ± 0.04	23	1.7
B7	B0 + V130T	36.5 ± 6	1.30 ± 0.08	36	55.8 ± 15.3	0.95 ± 0.11	17	2.1
B8	B0 + V55F	188 ± 13	2.65 ± 0.06	14	391 ± 21	2.56 ± 0.06	6	2.3
B6-II	B0 + V130T, L191A	99.3 ± 4.9	6.21 ± 0.09	63	158 ± 13	2.82 ± 0.09	18	3.5
B8-II	B0 + V55F, V130T	91.6 ± 12.5	1.34 ± 0.07	15	210 ± 31	0.91 ± 0.07	4	3.8
B6-III	B0 + V55F, V130T, L191A	252 ± 34	2.40 ± 0.10	9.5	632 ± 31	2.10 ± 0.10	3	3.2