TABLE 1.
Genes upregulated in sym1Δ versus wild-type cells on ethanol at 37°Ca
| Gene | Function | Fold induction | Category |
|---|---|---|---|
| PDC5 | Pyruvate decarboxylase | 14.5, 4.0 | Metabolism |
| PYC2 | Pyruvate carboxylase | 5.1, 2.1 | Metabolism |
| PGI1 | Glucose-6-phosphate isomerase | 2.6, 2.0 | Metabolism |
| SDH2 | Succinate dehydrogenase | 2.6, 2.9 | Metabolism |
| NTH2 | Alpha, alpha-trehalase | 2.4, 2.7 | Metabolism |
| BAP2 | Amino acid transporter | 2.6, 2.1 | Metabolism |
| ACH1 | Acetyl-CoA hydrolase | 2.6, 2.1 | Metabolism |
| SSC1 | Hsp70-mitochondria | 5.5, 2.9 | Heat shock |
| SSA1b | Hsp70-cytosolic | 2.3, 2.3 | Heat shock |
| FKS1 | 1,3-Betaglucan synthase | 5.3, 2.0 | Cell wall |
| PIR1 | Cell wall structural protein | 4.8, 2.5 | Cell wall |
| PMA2 | Plasma membrane ATPase | 4.5, 2.6 | Other |
| YCL042Wc | Uncharacterized ORF | 3.4, 2.1 | Other |
| YAL053W | Uncharacterized ORF | 2.1, 2.1 | Other |
a
Wild-type and sym1Δ cells were grown overnight at 30°C in glucose medium, shifted to 30°C and grown to mid-logarithmic phase in ethanol as sole carbon source. Cultures were then split and either continued growth at 30°C or shifted to 37°C until growth arrest. RNA was isolated and transcriptional profiles generated comparing the two strains at each temperature. The induction ratios in sym1Δ versus wild-type cells for genes upregulated twofold or more in both replicates are listed.
b
SSA1 was also independently found as the overlapping predicted ORF YAL004W.
c
Hypothetical ORF partially overlapping the glucokinase gene GLK1.