Figure 3.

Reversibility of ebselen inhibition of a HCV helicase–DNA interaction. (A) NS3h (10 μM) was incubated with the indicated concentrations of ebselen and diluted such that it could be added to Cy5-dT15, FP was measured, and activity remaining was calculated by comparing ebselen containing assays to controls not treated with ebselen. (B) Fluorescence polarization of Cy5-dT15 (5 nM) at different concentrations of NS3h, which was dialyzed following incubation with 100 μM ebselen (circles) or 0.5% v/v DMSO (triangles). Points are the averages of values obtained from two independent assays, and the error bars are the standard deviations.