TABLE 1.
Structural characteristics of the amoebae used in this study
| Amoebae | No. of vacuoles/cella | No. of mitochondria/cell |
|---|---|---|
| Wild type | 7.8 ± 2.5 | 9.2 ± 2.7 |
| Ac3R | 4.5 ± 1.9b | 12 ± 4.3b |
| Ac4R | 4.4 ± 2.2b | 11 ± 3.5 |
| Ac6R | 4.1 ± 2.2b | 11 ± 5.3 |
| Ac9R | 4.5 ± 2.1b | 15 ± 4.4b |
| 6-mo wild type | 6.1 ± 1.5 | 9.3 ± 2.6 |
| 6-mo Ac3R | 4.2 ± 1.2b | 14.4 ± 3.3b |
| 6-mo Ac4R | 4.8 ± 1.2b | 11.4 ± 2.7 |
| 6-mo Ac6R | 5.1 ± 1.5b | 12.0 ± 3.1 |
| 6-mo Ac9R | 4.8 ± 1.4b | 14.8 ± 2.9b |
a
Number of food vacuoles (excluding contractile vacuole) per amoeba in two counts of 50 cells each from amoebal variants and the wild type immediately after isolation and following passage in the laboratory for 6 months. The results are the means ± standard deviations. The data are representative of at least two independent experiments.
b
Significantly different (P < 0.05) from the result for wild-type A. castellanii.