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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Biomaterials. 2013 Feb 4;34(13):3231–3245. doi: 10.1016/j.biomaterials.2013.01.031

Figure 4. MSC cell growth is similar in the different sterilized and stored de-cellularized lungs except for the 6 month storage condition.

Figure 4

Representative H and E images of de-cellularized lungs treated under different storage and sterilization techniques and intratracheally inoculated with MSCs are shown. Images demonstrate characteristic re-cellularization one day after inoculation for all conditions (left). Robust re-cellularization was maintained for 28 days in culture (right) for all conditions except for de-cellularized lungs stored for 6 months in which no viable cells were observed after 7–14 days in culture. a = airways, bv = blood vessels. Arrows indicate areas where the MSCs have migrated out of the de-cellularized lung slices and formed agglomerates on plastic of the tissue culture dish. N = 3 for each condition (N = 2 for the 6 month storage condition). Original magnification 100X.