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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Biomaterials. 2013 Feb 4;34(13):3231–3245. doi: 10.1016/j.biomaterials.2013.01.031

Table 1.

Statistical analysis of the mass spectrometry data from the different sterilization and storage conditions for de-cellularized mouse lungs.

Values represent p-values from two group comparisons of raw unique peptide hits using the non-parametric test (pre-planned, selected, pairwise comparison of treatment group versus young decellularized lung controls). Protein group assignments were generated from UniProt entries of positively identified proteins (proteins containing two or more unique peptide hits). Grayed boxes with italicized text indicate the treatment group (labeled in the top row) reached statistical significance (p-values less than 0.05) with higher amounts of unique peptide hits than the control group (young de-cellularized lungs). White boxes with black, bolded text indicates the control group contained significantly more unique peptide hits.

Gene Protein Name Cold 3mos 6mos Irrad Para IPI
Cytoskeletal
Acta1 Actin, alpha skeletal muscle 0.026 0.060 1.000 0.155 0.262 IPI00110827.1
Cbr2 Carbonyl reductase [NADPH] 2 0.002 0.321 0.214 0.321 0.012 IPI00128642.1
Flna Isoform 1 of Filamin-A 0.550 0.012 0.036 0.107 0.071 IPI00131138.10
Msn Moesin 1.000 0.286 1.000 0.083 0.024 IPI00110588.4
Tuba1a Tubulin alpha-1A chain 0.002 0.131 0.429 0.274 0.036 IPI00110753.1
Tuba1b Tubulin alpha-1B chain 0.182 1.000 1.000 1.000 0.012 IPI00117348.4
Tubb2b Tubulin beta-2B chain 0.009 0.202 0.536 0.345 0.131 IPI00109061.1
Extracellular Matrix Proteins
Agrn_1 Isoform 2 of Agrin 0.455 0.012 1.000 0.012 0.083 IPI00378698.6
Col1a1 Isoform 1 of Collagen alpha-1(I) chain 1.000 0.500 1.000 0.500 0.012 IPI00329872.1
Col1a2 Collagen alpha-2(I) chain 0.002 0.012 0.036 0.012 0.012 IPI00222188.4
Col4a1 Collagen alpha-1(IV) chain 1.000 1.000 1.000 1.000 0.012 IPI00109588.4
Col4a2 Collagen alpha-2(IV) chain 0.517 0.702 0.714 0.214 0.024 IPI00338452.3
Col4a3 Collagen alpha-3(IV) chain 1.000 1.000 1.000 1.000 0.012 IPI00137938.7
Col6a1 Collagen alpha-1(VI) chain 0.004 0.155 0.071 0.060 0.024 IPI00339885.2
Col6a2 Collagen alpha-2(VI) chain 0.732 0.060 0.071 0.012 0.012 IPI00621027.2
Col6a3 collagen alpha-3(VI) chain 0.734 0.012 0.143 0.012 0.060 IPI00830749.3
Emilin1 Emilin-1 0.006 0.012 0.036 0.107 0.214 IPI00115516.1
Fbn1_1 Fibrillin-1 0.002 0.012 0.250 0.012 0.012 IPI00122438.1
Fbn1_2 Fibrillin -1 0.015 0.012 0.250 0.012 0.083 IPI00338565.2
Fn1_1 Fibronectin 0.818 0.024 0.321 0.012 0.095 IPI00113539.2
Hspg2_1 Basement membrane-specific heparan sulfate proteoglycan core protein 0.074 0.798 0.179 0.012 0.012 IPI00113824.1
Lama3_1 Isoform B of Laminin subunit alpha-3 0.039 0.024 0.893 0.869 0.036 IPI00125058.2
Lama4 Laminin subunit alpha-4 0.002 0.143 0.036 0.107 0.250 IPI00223446.5
Lama5 Laminin subunit alpha-5 0.054 0.024 0.393 0.095 0.012 IPI00116913.3
Lamb1 laminin subunit beta-1 0.004 0.750 0.036 0.036 0.048 IPI00338785.3
Lamb2 laminin subunit beta-2 precursor 0.011 0.012 0.321 0.500 0.012 IPI00109612.2
Lamc1 Laminin subunit gamma-1 0.199 0.012 0.679 0.488 0.262 IPI00400016.1
Lamc2 laminin subunit gamma-2 0.792 0.202 0.071 0.012 0.036 IPI00117115.3
Myh10 Myosin, heavy polypeptide 10, non-muscle 0.006 0.012 0.036 0.726 0.857 IPI00338604.5
Myh11_2 myosin-11 isoform 1 1.000 0.012 1.000 0.333 0.012 IPI00938530.1
Myh6 Myosin-6 0.006 0.321 0.071 0.060 0.845 IPI00129404.1
Myh9 Myosin-9 0.121 0.012 0.036 0.012 0.369 IPI00123181.4
Nid1 Nidogen-1 1.000 0.214 0.250 0.024 0.071 IPI00111793.1
Intracellular cytoplasmic
Actb Actin, cytoplasmic 1 0.024 0.060 0.250 0.512 0.024 IPI00110850.1
Alb Serum albumin 0.002 1.000 1.000 1.000 1.000 IPI00131695.3
Aldh2 Aldehyde dehydrogenase,mitochondrial 0.015 1.000 1.000 1.000 1.000 IPI00111218.1
Des Desmin 0.043 0.012 0.071 0.012 0.131 IPI00130102.4
Epx Eosinophil peroxidase 0.019 0.167 0.357 0.167 0.476 IPI00113854.1
Hadha Trifunctional enzyme subunit alpha, mitochondrial 0.061 1.000 0.036 0.012 0.012 IPI00223092.5
Hbaa1 Putative uncharacterized protein 0.002 0.393 0.607 0.119 0.393 IPI00110658.1
Hbbb1 LOC100503164 Beta-globin 0.002 0.083 0.643 0.429 0.083 IPI00762198.2
Hbbb2 Hemoglobin subunit beta-2 0.002 1.000 1.000 1.000 1.000 IPI00316491.4
Idh2 Isocitrate dehydrogenase [NADP], mitochondrial 1.000 1.000 1.000 0.012 1.000 IPI00318614.9
Myo1c Isoform 2 of Myosin-Ic 0.013 0.012 0.250 0.012 0.036 IPI00467172.2
Prg2 bone marrow proteoglycan 0.028 1.000 1.000 1.000 1.000 IPI00126365.1
Tgm2 Protein-glutamine gamma- glutamyltransferase 2 0.002 0.024 0.679 0.024 0.060 IPI00126861.3
Intracellular nuclear
Eef1a1 Elongation factor 1-alpha 1 0.002 1.000 0.250 1.000 0.333 IPI00307837.6
Eef1a2 Elongation factor 1-alpha 2 1.000 1.000 0.036 0.083 1.000 IPI00119667.1
Hist1h2bf_j_l_n Histone H4 0.006 0.012 0.286 0.060 0.012 IPI00407339.7
Hist2h2ac_b Histone H2A type 2-C 0.002 1.000 1.000 1.000 1.000 IPI00272033.3
Hnrnpk Isoform 2 of Heterogeneous nuclear ribonucleoprotein K 0.030 0.060 0.107 0.083 0.131 IPI00224575.1
Ptrf Polymerase I and transcript release factor 0.015 0.333 0.036 0.012 0.012 IPI00117689.1
Membrane
Atp5a1 ATP synthase subunit alpha, mitochondrial 0.255 0.071 0.179 0.071 0.036 IPI00130280.1
Atp5b ATP synthase subunit beta, mitochondrial 0.017 0.881 0.429 0.524 0.012 IPI00468481.2
Ehd2 EH domain-containing protein 2 0.006 0.012 1.000 0.452 0.119 IPI00402968.1
Iqgap1 Ras GTPase-activating-like protein IQGAP1 0.006 0.488 0.071 0.024 0.429 IPI00467447.3
Mfap4 Isoform 1 of Microfibril-associated glycoprotein 4 0.182 0.333 0.036 1.000 1.000 IPI00133751.1
Npnt Isoform 4 of Nephronectin 0.002 0.012 0.250 0.012 0.012 IPI00124689.1
Slc4a1 Isoform Erythrocyte of Band 3 anion transport protein 0.002 1.000 1.000 1.000 1.000 IPI00120761.3
Tln1 Talin-1 0.907 0.012 0.107 0.131 0.095 IPI00465786.3

italics treatment group significantly higher

gray rll significantly higher