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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Vox Sang. 2012 Feb 20;103(2):137–144. doi: 10.1111/j.1423-0410.2012.01590.x

Figure 2. Immunoblot analysis of AQP1 from RBC membrane lysates prepared from the proband.

Figure 2

Equal amounts of membrane lysates prepared from the RBCs of two control donors (lanes 1 and 4), a previously characterized Conull subject (SAR, lane 2) and the proband (lane 3) were resolved by Tris-Glycine 12% SDS-PAGE electrophoresis. Samples were not reduced or heat-denatured prior to electrophoresis, and were transferred to PVDF membrane for immunoblot analysis.

(A) PVDF membrane probed with goat anti-AQP1 (upper panel) and re-probed with mouse anti-GAPDH (lower panel).

(B) PVDF membrane probed with rabbit anti-AQP1 (upper panel; the smear migrating between 37 and 55 kDa corresponds to glycosylated AQP1) and re-probed with mouse anti-dematin (lower panel; erythroid dematin is expressed as two isoforms produced by alternative splicing).

(C) PVDF membrane probed with mouse anti-AQP1 (upper panel) and re-probed with rabbit anti-p55 (lower panel).