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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Oncogene. 2012 Jan 16;31(43):4609–4618. doi: 10.1038/onc.2011.606

Figure 2. Notch1 affects NRG1 expression.

Figure 2

A). Quantitative real time PCR on WM266-4 cells (cell line # 12 in Fig. 1G) expressing two shRNA sequences against Notch1. Notch1 and NRG1 mRNA levels were normalized with β-actin mRNA. B). Representative western blot for Notch1-TM, NRG1 and β-actin in WM266-4 cells expressing the shNotch1-2. C). V2387 (cell line #4 in figure 1G) expressing active Notch1 (NIC). Both Notch1 and NRG1 mRNA levels are reported after normalization with β-actin. D). Western blot analysis for Notch1-NIC and NRG1 in V2387 cells expressing an empty lentiviral vector (pLM) or the active Notch1-NIC (pLM-NIC). β-actin was used as a loading control. The data shown are representative of at least three independent experiments.