FIG. 3.

Disruption of the kipB gene. (A) Schematic of the strategy. The nutritional marker gene argB was inserted into kipB and thereby deleted 18 bp. (B) Southern blot analysis of a ΔkipB disruption strain (SPR13). Genomic DNAs of the wild type (FGSC26) and the kinesin disruptant strain were isolated, digested with SacI (left) and EcoRV (right), separated in a 1% agarose gel, blotted, and hybridized with the probe shown in panel A. (C) Effect of different benomyl concentrations on colony growth of wild type (RMS011) and ΔkipB disruptant (SPR13). Strains were inoculated on agar plates at 37°C and supplemented with the indicated benomyl concentrations dissolved in dimethyl sulfoxide.