TABLE 2.
Plasmids used for this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pBluescript KS(−) | Cloning vector | Invitrogen (NV Leek, The Netherlands) |
| pCR2.1-TOPO | TA cloning vector for cloning of PCR fragments | Invitrogen (NV Leek, The Netherlands) |
| pCMB17apx | alcAp::GFP pyr4; used for N-terminal fusion of GFP to proteins of interest | V. Efimov (Piscataway, N.J.) |
| pDC1 | A. nidulans argB gene in pIC20R | 2 |
| pHW-arg | KpnI-XhoI-released argB from pDC1 inserted into pBluescript KS(−) | 44 |
| pRG1 | Contains N. crassa pyr4 gene | 43 |
| pPR5 | kipB containing cosmid (BamHI short fragment, 3.3 kb) from pU1 library | This study |
| pGR1 | Subclone BamHI short fragment of kipB from pPR5 into pBluescript KS(−) | This study |
| pPR7 | 3.6-kb SacI-BamHI fragment of kipB cloned into pBluescript KS(−) | This study |
| pPR11 | alcAp::kipB::sgfp (kipB BamHI fragment from pPR7) cloned into pBluescript KS(−) | This study |
| pPR13 | argB gene with BamHI sites cloned into BglII sites of kipB from pPR7 (disruption construct) | This study |
| pPR17 | kipB containing cosmid (entire gene) from pU1 library | This study |
| pPR19 | 4.3-kb kipB SacI subclone from pPR17 cloned into pBluescript KS(−) | This study |
| pPR38 | 1.2-kb kipB fragment from ATG, PCR product with AscI and PacI sites from pPR19 inserted into pCMB17apx | This study |
| pPND1 | GFP replaced with mRFP1 (KpnI-AscI) into pPR38, alcA(p)::mRFP1::kipB, pyr4 | This study |