Table A2.
Tissue enrichment of the differentially expressed (DE) genes among polyandrous (E) and monandrous (M) females. The expected numbers are based on the numbers of genes enriched in each tissue in the FlyAtlas dataset. Deviation from 1:1: tests for differences in the relative proportion of genes up-regulated in polyandrous and monandrous females out of all DE genes enriched in that tissue. For example, there are 165 differentially expressed genes that are enriched in the testis, of which 121 are up-regulated in E and 44 in M. This test compares whether their respective proportions out of the total number of 165 genes differ from one another
| TEST | DE genes with higher expression in E | DE genes with higher expression in M | Deviation from 1:1 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tissue | Obs. | Exp. | χ2 | Bonf P-value | Obs. | Exp. | χ2 | Bonf P-value | χ2 | Bonf P-value |
| Accessory glands | 121 | 140 | 3.2 | n.s. | 80 | 84 | 0.15 | n.s. | 15.9 | 0.001 |
| Adult fatbody | 94 | 122 | 8.2 | 0.07 | 1151 | 76 | 24.0 | 1.62E-05 | 3.8 | n.s. |
| Brain | 139 | 177 | 10.8 | 0.02 | 130 | 108 | 5.8 | n.s. | 0.5 | n.s. |
| Crop | 100 | 136 | 12.0 | 0.009 | 1201 | 84 | 18.6 | 0.0002 | 3.3 | n.s. |
| Eyes | 93 | 139 | 19.7 | 0.0002 | 1431 | 88 | 43.1 | 8.80E-10 | 20.3 | 0.0001 |
| Head | 77 | 157 | 53.6 | 4.08E-12 | 2001 | 102 | 121.8 | 4.39E-27 | 107.5 | 5.98E-24 |
| Heart | 89 | 104 | 2.4 | n.s. | 1211 | 65 | 56.7 | 8.63E-13 | 9.2 | 0.04 |
| Hindgut | 100 | 126 | 6.6 | n.s. | 1471 | 80 | 70.4 | 8.24E-16 | 17.1 | 0.0006 |
| Spermatheca mated | 89 | 123 | 11.8 | 0.01 | 110 | 77 | 17.5 | 0.0005 | 4.0 | n.s. |
| Midgut | 95 | 122 | 7.4 | n.s. | 1371 | 77 | 57.2 | 6.70E-13 | 14.5 | 0.002 |
| Ovaries | 2151 | 155 | 30.5 | 5.60E-07 | 26 | 85 | 51.3 | 1.36E-11 | 293.3 | 1.61E-64 |
| Salivary gland | 172 | 194 | 3.3 | n.s. | 116 | 116 | 0.002 | n.s. | 21.0 | 7.78E-05 |
| Thoracic ganglion | 124 | 165 | 13.7 | 0.004 | 1301 | 102 | 10.1 | 0.02 | 0.2 | n.s. |
| Testis | 121 | 162 | 13.7 | 0.004 | 44 | 95 | 35.2 | 5.09E-08 | 70.0 | 1.00E-15 |
| Tubule | 113 | 113 | 0.00007 | n.s. | 92 | 72 | 9.6 | n.s. | 3.9 | n.s |
| Trachea | 122 | 120 | 0.02 | n.s. | 871 | 68 | 3.7 | 0.03 | 11.1 | 0.01 |
| Spermatheca virgin | 90 | 115 | 6.4 | n.s. | 1031 | 71 | 17.4 | 0.0005 | 1.5 | n.s. |
Indicates overrepresentation in tests for E and M.
Bold text highlights the female selection type that shows significantly higher relative proportion of up-regulated genes in the focal tissue.